Category: writing

Friday, 27 January 2012

08:09 – I should finish the lab session on simulated DNA gel electrophoresis today and get it off to the reviewers. Finish in the sense of finish writing it. I haven’t actually done the lab session yet. I’ll do it this weekend, when we’ll also be shooting a lot of images for it and other lab sessions. That leaves only the introduction, which is in progress. It looks like we’re actually going to make the 31 January deadline.

Not that things settle down much after that. In addition to a flow of of queries and edits, I have to get the kit contents finalized and purchase orders cut for components. The biology book hits the bookstores in April, and by that time I want to have 60 finished biology kits in inventory and ready to ship, along with components in the pipeline for many more.

The next project is a complete re-write of the forensics lab book that we finished a couple of years ago but was never published. I want to rewrite that book around a custom kit, which will make it much more accessible to home schoolers and hobbyists. I’d like to have that book complete, at least in PDF form, and kits available in time for summer session, although realistically it’ll probably be in time for autumn semester.

Farther out, but still on the horizon, I’d like to do a second edition of Illustrated Guide to Home Chemistry Experiments, this one kit-based. Two kits, actually. One for first-year labs (the current chemistry kit) and one for second-year.

It’s going to be a busy year.


Read the comments: 37 Comments

Thursday, 26 January 2012

08:44 – I sent off the preface and lab safety chapter to the reviewers yesterday. That leaves the introduction, which is well in progress, and a lab session that I wasn’t sure I’d have time to do before deadline. That one is on simulated DNA gel electrophoresis, “simulated” because we’ll use dyes rather than actual DNA fragments for the separation.

Those dyes–crystal violet, methylene blue, and safranin O–are included in the kit as biostains, and they all migrate the same direction in a gel. We’ll use their different molecular masses as proxies for DNA fragments of differing BP size. We’d use real DNA, but that’d require expensive restriction enzymes, and wouldn’t produce very distinct banding in an agar gel (as opposed to agarose, which is much, much more expensive than agar, which isn’t cheap itself). I was thinking about having readers pre-run the gels to clear out some of the gunk that’s present in plain agar, but that’s really more trouble than it’s worth.


Read the comments: 25 Comments

Wednesday, 25 January 2012

07:49 – Heads-down writing continues, as it will for the next several days.

I was just reading an article to Colin yesterday about new evidence that dogs have been man’s best friend for at least 33,000 years, back when we were still Cro Magnon. Two skeletons of what are unquestionably domesticated dogs have been reliably dated to 31,000 BCE. To put that in perspective, it wasn’t until about 11,000 BCE that man domesticated the sheep, pig, and cow, and it wasn’t until about 6,000 BCE that women domesticated men.


Read the comments: 28 Comments

Tuesday, 24 January 2012

10:18 – The chapter on Equipping Your Lab is finished and off to the reviewers. I’m working on the Preface now.


15:29 – I’m still working on the Preface, but I did finish writing the Dedication.

To Charles Darwin (1809 – 1882), a towering genius whose theory of evolution is the foundation of modern biology.

Read the comments: 28 Comments

Monday, 23 January 2012

11:12 – With eight days left until deadline, I’m in serious heads-down writing mode. The lab sessions are complete, with one exception, and I’m working on the narrative early chapters now. There are four of those: Preface, Introduction, Equipping Your Lab, and Lab Safety. I’m working on the Preface now. The chapter on Equipping is essentially complete. It’s currently 51 manuscript pages and about 25,000 words, and I want to cut that down some. Lab Safety is reasonably complete, but needs a few more hours work. The Intro is the one that’ll take me two or three days to finish. So I’d better get back to work.


Read the comments: 2 Comments

Friday, 20 January 2012

10:14 – I’ll finish my final pre-editing pass on the lab sessions today and get them off to the reviewers. At that point, I jump back into the early narrative chapters to do clean-up and rewrite. I should finish that in the next few days. I also have a dozen or so images left to be shot, which I’ll do this weekend. So, for the next ten days or so, I’ll be busier than the proverbial one-armed paper hanger, incorporating edits and comments from reviewers and getting the manuscript ready to go to production on 31 January.

I was about to order gelatin and yeast on-line, until I realized that it’d be cheaper and faster just to make a visit to the supermarket. Sure enough, the local Lowes Foods has yeast for $0.59 for eight packets of 8.75 grams each, so I’ll just buy enough of those to make the first batch of kits. Same thing for gelatin, cheap and readily available. I’ll just buy a couple pounds of unflavored gelatin and repackage it for the kits.


12:52 – I’m uploading the last of the lab session chapters to the server right now. That takes a while, given that Time-Warner caps upload speeds around 125 KB/s (still an improvement over the 45 to 50 KB/s we got until a few months ago) and some of these chapter directories are rather large. Even with thumbnailed images, some of the chapters are 10 to 15 MB, and the scores of high-res images tend to add up. I think this batch totals something like 1.5 GB.

I’m going to reward myself by taking a ten-minute break and then jump back into the early narrative matter. Most of that doesn’t require tech review, so I wanted to get the lab session chapters available first to the reviewers.

I’m in my usual worry mode now. For some reason, I always think that the material I’ve submitted is going to end up after formatting and lay-out as a 30-page pamphlet or something. Of course, that’s ridiculous. No book I’ve ever written has come in under the allotted page count, and some have been significantly larger. Oh, well. I covered what I wanted to cover, and soon it’ll be on to building biology kits and starting on the re-write of the forensics book. I already have an idea for a lab session I want to add to that, but I’ll have to do some experiments to see if it’s practical. (Hint: it involves raw meat and flies.)

Read the comments: 24 Comments

Thursday, 19 January 2012

08:46 – I’m in heads-down mode on the biology book chapters, doing a final pass on them and sending off several a day to the editors. I’m also updating and annotating the bill of materials for the biology kits as I go along.

Some of the BoM changes are pretty trivial. For example, we’ll use ordinary unflavored gelatin in a couple of lab sessions. Until now, I’d included gelatin in the You-Provide list, but yesterday I decided to make it an Included-in-the-kit item. It’s certainly no real hardship to have kit purchasers buy a pack of unflavored gelatin at the supermarket, but including it means there’s just one less thing for them to worry about.

Other BoM changes have implications. For example, one of the lab sessions involves growing lima beans with and without Rhizobium, which is a nitrogen-fixing bacteria, and then comparing growth in the test specimens grown with and without nitrogen. That’s an interesting experiment, but the problem is that I can ship kits with the Rhizobium to 49 states, but not Hawaii. (Apparently, Hawaii is worried about a pestilence of nitrogen-fixing bacteria.) So, I had to update the BoM to make two kit versions, one for Hawaii and one for the other 49 states. That raises the issue of what Hawaii residents should do for this lab session. I decided to include a bottle of ammonium nitrate with only those kits shipping to Hawaii. Geez.


Read the comments: 30 Comments

Wednesday, 18 January 2012

09:35 – I was going to black out this site today in sympathy with the SOPA/PIPA protests, but I couldn’t figure out how to turn the whole page black. If SOPA/PIPA does pass, I’ll have to make some changes around here, starting with disabling comments.


I’m still cranking away on my final pre-editing pass of the lab sessions. There are quite a few missing images, which I’m just putting in placeholders for for now. I created a to-be-shot images list, and I’ll go through and shoot those in a batch.


USPS introduces a new regional-rate box today, to join the current RR Boxes A and B. The new RR C box is considerably larger—12x12x15” (30.5×30.5x38cm)—and might actually have been useful had the USPS not priced it ridiculously high.

The smallest RR box, A, requires postage at the Priority Mail 2-pound level, and can be used for up to 15 pounds. The mid-size RR box, B, which is what I use for kits, requires postage at the Priority Mail 4-pound level, which means it costs me $5.81 in postage for relatively nearby destinations up to $14.62 for zone 8 (the west coast, Hawaii, and Alaska). It can be used for up to 20 pounds. The new RR Box C is priced at the Priority Mail 17-pound level, which means it would cost almost $15 to send to nearby destinations and about $45 to send to zone 8. It can be used for up to 25 pounds. That’s not a very good deal, considering that the large flat-rate PM box (12x12x5.5”, up to 70 pounds) costs only about $15 to send to any address in the US, including zone 8.

There’s a lot of discussion about this new box on the forums frequented by eBay sellers and other vendors. Many people thought the 17-pound rate was a typo, and that USPS really meant to say the 7-pound rate. That might have been reasonable, but as it turns out they really did mean the 17-pound rate. In effect, the USPS has made this new box useless other than for a very small percentage of shipments: those that weigh between 18 and 25 pounds, are too large to fit a large flat-rate box, and are going to distant addresses. Otherwise, it’s cheaper to use UPS or FedEx. Sometimes far cheaper.

If USPS had been smart, they’d have made the RR box C a 12x12x10” box with a 25-pound limit and priced it at the PM 7-pound rate. That box would have been very useful and very widely used. But a RR box C that costs from $14+ to $45 is simply a non-starter.


Read the comments: 35 Comments

Tuesday, 17 January 2012

08:11 – I just mailed off the state and federal estimated tax payments. Government should be just like any other product. If you want it, you pay for it voluntarily. If you don’t, you don’t.

I spent yesterday working on the front matter for the biology book and also on rewrite on the first group of lab sessions. I just re-worked one of those, on antibiotic sensitivity of bacteria, yet again. This is the third time, so let’s hope it’s a charm. In the first iteration, I based the lab session around antibiotic test papers, which is the usual method of testing sensitivity of bacteria to different antibiotics.

The problem with that was that I wanted to do a follow-up procedure in which we retested the now-resistant bacteria against the various antibiotics. That required re-culturing the bacteria in the presence of an antibiotic, which really meant I needed to supply the antibiotics in more concentrated form. So, several weeks ago, I rewrote that procedure to use solutions of the antibiotics rather than impregnated test papers, and respecified the kit contents accordingly.

The problem with that method is that it turns out that dilute solutions of some of the antibiotics I used are not stable in dilute solutions, even when refrigerated. So, yesterday I re-rewrote that procedure around different antibiotics, one of which I can supply with the kits in liquid form, one as capsules, and two as powders, all of which are stable.

That, of course, meant that I had to track down sources for the materials, put together purchase orders, and actually order the materials for the first batch of kits. Not to mention creating new labels for those components and creating new MSDS sheets for each. I’ve now done all that (other than the MSDSs), and updated the bill-of-materials for the kits.


14:12 – Wow. I just took a break to do a backup, and while the backup is copying to the thumb drives I was checking news sites. It appears that that Italian cruise-ship company has hit the rocks in more ways than one. The ship itself is a write-off, and I suspect the insurers will balk at paying a third of a billion dollars to replace the ship, given that the captain acted completely irresponsibly. I understand that he knowingly approached the rocks to get close enough to shore that his head cook could wave to a family member. Then the captain bailed at his first opportunity. The hell with his passengers, he was out to save himself. And now I see that the company has left the shipwrecked passengers stranded, literally. Most of the passengers lost all of their possessions, including passports, credit cards, and cash. Not to mention things like prescription medications. Ordinarily in a situation like this, one would expect the company to fall all over itself to help the people it dumped into the ocean. Buy them clothing and personal items to tide them over. Intercede to help them get temporary papers or replacement passports. Pay airfare to get them back home. And so on. Apparently, this company is doing literally nothing to help, not so much as buying the victims a cup of coffee. Victims who’ve asked for help have been told to sue the company. It’s not that the company is trying to avoid admitting responsibility. They’ve already done that. They’re just not willing to spend a cent to help the victims. Apparently, the captain was not the only miserable excuse for a human being employed by this company.

Read the comments: 42 Comments

Monday, 16 January 2012

08:22 – I finished the lab session on vertebrate tissues yesterday and sent it off. Today, I jump back to the first lab session and start working my way forward, cleaning up as I go.


Read the comments: 31 Comments
// ------------------------------------------------------------------------------- // end of file archive.php // -------------------------------------------------------------------------------